Gradient LC analysis of herbicides and polyaromatic hydrocarbons by isocratic capillary electro- chromatography

Capillary electrochromatography (CEC) combines the separation principle of HPLC (partitioning between mobile and stationary phases) with the high efficiency of capillary electroseparation methods. In CEC the electroosmotic flow (EOF) inherent in capillary electrophoretic separations is used to transport solute and mobile phase through a packed capillary column. The properties of the EOF provides higher efficiencies than can be realized with LC. This can be sufficient to allow the transfer of methods conventionally performed by gradient LC to be performed by isocratic CEC. Authors Gordon Ross, Monika Dittmann Agilent Technologies, Waldbronn, Germany Thomas Adam University of Mainz, Germany Application Note